Establishment of <i>Echinococcus granulosus EgM123</i> Recombinant Gene Rabies Virus SRV<sub>9</sub> and Identification of Its Biological Characteristics.

The viral mRNA expression levels were recorded as 0.97, 1.09, 1.611, 1.287, and 1.699, respectively, and viral mRNA expression levels showed an increasing trend, suggesting that the EgM123 recombinant gene RV was successfully rescued; the three-day-old suckling rat LD 50 assay determined that the recombinant virus titer was 10 −1.4 /0.03 mL and the original virus titer was 10 −6.7 /0.03 mL [ 30 ], suggesting that the recombinant virus constructed by reverse genetics approaches had a weaker virulence compared with that of the parental SRV 9 strain.

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