Topic 2 (physiology) showed a decreasing trend from the 1990s to the 2010s, followed by an increase from 2015 onwards.
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Trends and Challenges in Plant Cryopreservation Research: A Meta-Analysis of Cryoprotective Agent Development and Research Focus.
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The sentences
Topic 3 (cryopreservation protocol) exhibited an increasing trend from the 1990s to 2005, remained steady until 2015, and began to decline in the 2020s. 3.