Single-cell transcriptomics reveals the multidimensional dynamic heterogeneity from primary to metastatic gastric cancer.

Notably, myeloid, epithelial, and stromal cells showed decreasing trends during PT-to-M progression, whereas B cells exhibited an increasing trend during GC lymph node metastasis progression, and T cells showed an increasing trend during both GC lymph node and liver metastasis progression ( Figures 1 A and 1B).

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Specifically, CD8 + Tem cells, neutrophils, monocytes, CD3/CD28-stimulated NK cells, endothelial cells, and CAFs subsets showed a decreasing trend during the PT-to-M progression.

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