Application of Staining Methods to Compare Chromatin Condensation in Fresh and Freeze-Thawed Dog Semen.

Reprod Domest Anim · 2025 · PMC11871417 · PMID 40022439

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closest p · 0.2× alpha

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highly significantp < 0.01actually significant

Table 2 outlines the statistical significance between different staining methods for fresh semen, where aniline blue showed highly significant differences ( p < 0.01) with Toluidine blue, AcO and CMA3, but no significant difference with TUNE.

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