However, functional gene set enrichment analysis (GSEA) of the global changes in gene expression between mildly and severely affected chickens, identified an interesting trend: Functions associated with inflammation and acute phase response were enriched with a positive Normalized Enrichment Score (NES) in severely WB-affected chickens, while functions associated with cell proliferation and metabolism were enriched with a negative NES ( Figures 6A–C ). 4 Discussion In the present study, we have investigated the histological and molecular alterations in the hearts of mildly and severely WB-affected chickens.
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