A p -value less than 0.05 indicated a significant (∗) difference, while a p -value less than 0.01 indicated an extremely significant (∗∗) difference. 3 Results and discussion 3.1 Effects of the five typical antimicrobial chemicals on the hatching rate of zebrafish Hatching is a key step in the transition from an embryo to a larval fish; therefore, the hatching rate is commonly used to evaluate whether a chemical harms the development and growth of an organism [ 44 ].
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Common antimicrobials disrupt early zebrafish development through immune-cardiac signaling.
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