highly significantp < 0.001
After 25 min of incubation, samples at concentrations of 0.01 ng/μL, 0.1 ng/μL, 1 ng/μL, and 10 ng/μL showed highly significant differences ( p < 0.001) compared to the control group (CK), while no significant difference was observed for the 0.001 ng/μL samples ( Figure 4 b).