Spatiotemporal analysis of ratiometric biosensors in live multicellular spheroids using SPoRTS.

Cell Rep Methods · 2025 · PMC11955269 · PMID 39965566

Europe PMC DOI

hedged sentence

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closest p · 0.0× alpha

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highly significantp = 1.1e−6actually significant

As we anticipated, Geminin staining was strongest in the region closest to the surface, and we found a strong and highly significant correlation between these results and those obtained from SPoRTS analysis of rFUCCI-expressing spheroids (Pearson’s r 2 adj = 0.9979, p = 1.1e−6; Figure 5 D).

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