Effects of dietary fiber on the composition, function, and symbiotic interactions of intestinal microbiota in pre-weaned calves.

Candidatus Gastranaerophilales showed a highly significant correlation with 5 GHs ( P < 0.01, R > 0.25), a significant correlation with 4 GHs ( P < 0.05), and a significant correlation with PL10.hmm and GT92.hmm ( P < 0.05, R > 0.25). 3.5 Impact of dietary fiber on gut microbial symbiosis in 56-day-old calves We analyzed the symbiotic relationships within the intestinal microbiota of 56-day-old calves and found that calves supplemented with inulin displayed weak intergenus associations, with only the Prevotella, Clostridium , and Bacteroides genera showing relatively strong intragenus correlations ( Figure 9A ).

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In contrast, calves supplemented with inulin displayed no statistically significant differences from the control group, although the alpha diversity indices exhibited an increasing trend ( Figure 2 ).

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