Hyphenation of microflow chromatography with electrospray ionization mass spectrometry for bioanalytical applications focusing on low molecular weight compounds: A tutorial review.

Mass Spectrom Rev · 2025 · PMC11976378 · PMID 38952056

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For microflow configurations, it is advisable to avoid transferring a single analyte plug from the trapping column with a strong solvent, as the volume of such a plug may be significant in comparison to the volume of the analytical column, potentially causing breakthrough.

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