Optimization of the enrichment medium for recombinant ChIL-4-ChIL-2 in Lactococcus lactis through response surface methodology.

After the elimination of the x 2 x 3 term, the optimal multiple regression equation for the response variable OD 600 was ultimately formulated as follows: y 1= 0.665478+0.315105 x 1 -0.0185 x 2 +0.596132 x 3 -0.029 x 1 x 2 +0.123368 x 1 x 3 -0.086 x 1 2 +0.005 x 2 2 -0.435333 x 3 2 The results of significance testing and variance analysis indicated that the p-value of the model was less than 0.0001, signifying that the equation model was highly significant and effectively accounted for the variability observed in the experimental data.

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Results and analysis Single-factor experiments Screening carbon sources The yields of recombinant ChIL-4-ChIL-2 in Lactococcus lactis in M17 with trehalose, lactose combined with glucose (GM17), glucose, lactose, and sucrose, exhibited a decreasing trend.

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