Single-cell genomics of single soil aggregates: methodological assessment and potential implications with a focus on nitrogen metabolism.

While the cavitation effect of ultrasound by sonication has been demonstrated to disrupt cell structure and associated chemical effects including the generation of free radicals can damage DNA ( Li et al., 2018 ; Lv et al., 2019 ; Miller et al., 1991 ; Oyane et al., 2009 ), these negative effects may not be significant.

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