Synteny Enabled Upgrade of the Galapagos Giant Tortoise Genome Improves Inferences of Runs of Homozygosity.

For both PLINK and RZooROH methodologies, we detected highly significant differences in F ROH , N ROH , and S ROH between genome versions ( p < 0.001 in all cases; Table 2 and Tables S4 and S5 ; Figure 1 ); specifically, CheloAbing_2.0 consistently produced smaller N ROH and greater F ROH and S ROH estimates than Chelo_Abing_1.0 across pairs of samples.

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