Enhanced nattokinase production by <i>Bacillus subtilis</i> from glycerol and okara: optimization of culture medium via response surface methodology.

A P -value of < 0.05 indicates a significant difference, while a P -value of < 0.0001 indicates an extremely significant difference. 3 Results 3.1 Fermentation of nattokinase activity by different substrates As illustrated in Figure 1A comparative analysis was conducted to evaluate the differences in enzyme production for nattokinase fermentation using various carbon substrates.

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