Additionally, we quantified expression of ruf6 , a non-coding RNA known to regulate transcription of the var gene family [ 62 ], and observed similar trends to those seen for var ATS quantification, albeit without statistical significance between any of the individual groups; and with borderline significant differences between ruf6 in clinical malaria cases (MAL) versus all asymptomatic samples combined ( Fig 4B ).
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Plasmodium falciparum expresses fewer var genes at lower levels during asymptomatic dry season infections than clinical malaria cases.
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