highly significantp < 0.001
leontopetaloides -derived compounds, the novel compound Tarkalynin A ( 1 ) displayed the most promising activity, but again with a highly significant separation between the Trypanozoon and Nannomonas subgenera ( p < 0.001), as EC 50 values against the former ranged between 21.4 μM ( T. equiperdum ) and 46.0 μM for T. evansi , whereas the compound was almost inactive against T. congolense (EC 50 = 246 μM).