Optimization of bioactive polyphenols recovery from Flammulina velutipes stem waste using nonionic surfactant-integrated ultrasound-assisted extraction.

In the case of TPC, the interactions and quadratic terms ( X 3 , X 1 X 2 , X 1 X 3 , X 1 2 , X 2 2 , X 3 2 , and X 4 2 ) were found to be extremely significant at p < 0.001, and the interaction terms ( X 4 , X 2 X 3 ) was notable at p < 0.05, whereas other variables had no marked effect on the TPC at a level of p > 0.05.

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