highly significantp ≤ 0.01
Statistical differences were considered significant if p -values were less than 0.05 (* p ≤ 0.05) and highly significant if p -values were less than 0.01 (** p ≤ 0.01), less than 0.001 (*** p ≤ 0.001), or less than 0.0001 (**** p ≤ 0.0001).