Integrative Physiology, Transcriptome, and Metabolome Analysis Reveals Pathways and the Key Gene <i>ZmARF27</i> Involved in Vigor Loss during Artificial Aging of Maize Seeds.

The mutation site was identified at 501 bp (G/A; Figure D). To validate the mutant zmarf27 , we examined its expression during the 24 h following germination after 4 days of artificial aging. In both the wild-type B73 and the mutant strains, the expression of zmarf27 exhibited an overall trend of increasing followed by decreasing, with a peak at 24 h postgermination ( Figure E).

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