Why AGG is associated with high transgene output: passenger effects and their implications for transgene design.

As regards agreement about the best codon for any amino acid at any position, using as above mean null concordance and doing one binomial test for the tiles overall, the P -value is again marginally significant (binomial test, 75 successes from 173 trials with weight mean null expectation p = 0.36: P = 0.048, two tailed, 62 expected under the null of no agreement; Supplementary Fig.

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In a linear model, all variables are highly significant (there are 232 833 degrees of freedom so even miniscule effects are highly significant) and explain 21.39% of the variation in protein per RNA.

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We find that comparing the enrichment of each codon in the 5′ domain of nHEGs against the enrichment seen in the Cambray data, there can be either a very weak positive or weak negative correlation, none even close to significance (Fig. 8 ).

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