First report on the infection of <i>Acanthamoeba</i> and <i>Entamoeba</i> in the endangered Chinese Milu deer (<i>Elaphurus davidianus</i>) in China.

For Entamoeba spp., population density emerged as a highly significant predictor of infection (OR = 11.71, 95 % CI: 3.79–36.12, p < 0.001), indicating that deer in high-density populations had substantially higher odds of testing positive for infection.

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