To gain specific information of the involvement of CENP-F in dynactin redistribution, we repeated similar experiments in cells subjected to CENP-F silencing; CENP-F-specific siRNAs yielded a highly significant decrease of the overall CENP-F cellular content, except for a small residual fraction persisting at spindle poles, while the kinetochores appear to be virtually devoid of CENP-F (Fig.
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The localisation and stability of the CENP-F protein are regulated by importin beta and microtubules in mitotic cells.
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