highly significantp = 2.2 × 10 −16
Moreover, the relationship between the two methods of load estimation was highly significant (F 1,72 = 2420, p = 2.2 × 10 −16 ), and the slope was not significantly different from 1 (0.96, 95% CI [0.97, 1.05]), supporting the hypothesis that only Wolbachia w Sc1 and w Sc2 circulate in the S. cleopatrae flea populations.