Improving the heterotrophic media of three Chlorella vulgaris mutants toward optimal color, biomass and protein productivity.

Overall, the factors that affected growth significantly were the concentration of N, P, Zn, Cu, B, Mn, Mo, Fe and vitamin solution and N source in the case of mutant 8G, the concentration of N, P, Ca, Cu and N source, and Zn was marginally significant for 7Y growth and the concentration of N, P, Ca, Mg, Cu, Zn, Ni and N source for mutant 31 W (Fig. 1 a and b).

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