Integrated transcriptomic and metabolomic analysis of flavonoid biosynthesis in cigar tobacco leaves under variable nitrogen regimes.

DEGs from the N0 and N1 treatments showed high similarity, which distinguished them from the other treatments, and most of the DEGs showed an increasing trend with increasing N application.

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Subcluster 1 contained 1,499 DEGs, which showed a decreasing trend with increasing nitrogen content.

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The results indicate a highly significant relationship among transcription factors, regulated genes, and metabolite expression.

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