highly significantP < 0.000
RESULTS: The observations made in the study suggested an increased number of microbial colonies in the NiTi closed-coil spring group (46437.50 ± 11848.34 CFU/mL) compared to the active tie-back group (31031.25 ± 8204.97 CFU/mL), with the difference being statistically highly significant ( P < 0.000).