highly significantp < 0.05
Combined NMDS and MRPP analyses revealed a highly significant difference in community structure between the L group and the other two groups ( p < 0.05) (ANOSIM, p = 0.008 and 0.028; MRPP, p = 0.009 and 0.015; Table 6 ). 3.4 Functional predictions in the fecal microbiota of female donkeys across different reproductive cycles We used PICRUSt to predict the functions in the fecal microbiota of female donkeys across different reproductive cycles and evaluated the accuracy of these predictions using the Nearest Sequenced Taxon Index (NSTI) values.