Betaine decreases hepatic lipid deposition through DNA 5 mC and RNA m<sup>6</sup>A methylation-mediated regulation of fatty acid metabolic genes expression in laying hens.

Since certain feed additives can modulate hepatic lipid deposition by regulating fatty acid metabolic genes expression ( Cai et al., 2016 ; Yan et al., 2022 ), strategically selecting additives that effectively alter these genes expression to reduce hepatic lipid accumulation is highly significant.

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