Total mixed ration enhances nutrient digestibility, blood biochemical parameters and faecal microbial diversity in horses.

A P -value < 0.05 was considered statistically significant, and a P -value < 0.01 was considered highly significant.

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In addition, this study observed an increasing trend in blood levels of Cre, T-Bil, D-Bil, TC, AST, AST/ALT, and CK in horses following TMR feeding.

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Notably, the abundance of Spirochaetota in the S group was significantly higher than in the C group ( P < 0.05), while Bacteroidota, Verrucomicrobiota and Proteobacteria abundance exhibited a decreasing trend in the S group.

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