an increasing trendp <0.001
The GnRH levels from NP-treated hypothalamus showed an increasing trend, especially significant in male 10 nM- and 100 nM-treated groups ( p <0.001) and female 1 nM-, 100 nM- and 1 μM-trated groups ( p <0.05, p <0.01 and p <0.05, respectively).