Alkaline phosphatase-streptavidin conjugate (APSA) enzyme and binding activity over time and storage conditions.

Vials of APSA stored on ice in the fridge for up to 22 months and then sampled at a dilution factor of 1/250,000 (DF 250,000) prior to assay showed extreme sample to sample variation in enzyme activity and a trend to lower OD values over time with a highly significant effect of the storage time on the detection limit (ANOVA p ≤ 2E-16, Regression p < 0.043) ( Fig. 2 A).

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Recently it was shown that the dissolution of glutaraldehyde cross linked proteins structures may be significant over days in aqueous environment [ 30 ].

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