As tested by in vitro germination and acetocarmine tests, the analysis of variance clearly showed that storage method, days of storage, and the interaction between storage methods and days of storage had a highly significant effect ( p ≤ 0.0001) on pollen viability.
Fruit quality in terms of TSS, DPPH and ABTS antioxidant assays showed a positive trend (highest at − 4 °C and − 20 °C), while total acidity and ascorbic acid content showed negative trends (lowest in − 20 °C) for low-temperature-stored pollen pollinated fruits as compared to fruits in room-temperature-stored pollen pollinated fruits.