Multi-endpoint assessment of tunnel wash water and tyre-particle leachate in zebrafish larvae.

A p-value of less than 0.05 was considered statistically significant (*), while a p-value of less than 0.005 was regarded as highly significant (**). 3 Results 3.1 Chemical characterization of tyre-wear pollutants Elevated levels of heavy metals such as Fe (865 μg/L), Zn (35 μg/L), Cu (10.3 μg/L) and Ni (3.57 μg/L) were found in UTWR water ( Supplementary Table 1 ).

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