Optimization of fermentation process for Sihuang compound with Bacillus subtilis and its efficacy against Escherichia coli infections and growth performance in broilers.

The regression fitting analysis yielded a polynomial regression equation: Y=246.40˗1.62 × A+4.38 × B+1.75 × C+9.50 × A × B˗4.75 × A × C+1.25 × B × C˗40.45 × A 2 ˗ 119.95 × B 2 ˗ 46.20 × C 2 The F -test was used to evaluate the statistical significance of the regression model, and the F -value of 161.05 was considered as the highly significant of the model ( Table S3 ).

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