Alterations in the Platelet Transcriptome Mediate Prenatal Thirdhand Smoke Exposure Associated Thrombogenicity via Integrated miRNA-mRNA Regulatory Networks.

Three genes were found to have borderline significant p values, whereas another gene followed the same trend as RNA seq without achieving significance and a separate one could not be identified by Q-PCR according to the qRT-PCR results of the coding genes, which corroborated the substantial down- or upregulation of 15 genes.

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With the exception of two miRNAs that maintained the trend with borderline significance and one miRNA that could not be detected by Q-PCR, the ten miRNAs chosen for Q-PCR validation also replicated the trend exhibited by the RNA seq data set. 2.8.

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