Stereological analysis of cholinergic neurons within bilateral pedunculopontine nuclei in health and when affected by Parkinson's disease.

PPN‐cholinergic neuronal quantification using this PPN delineation method resulted in a mean unilateral PPN‐cholinergic cell count in PD brains of 17,936 ± 1905, again resulting in a highly significant statistical difference when compared to counts made in control brains (**** p < 0.0001) and correlating with a 50% PD‐induced loss of cholinergic neurons.

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