highly significantP < 0.001
We found that the paired subsamples had highly significant positive correlations in VAF (%), as shown for #M42 (R 2 = 36.7, P < 0.001; Fig 2B ), which we present because it was the most widespread de novo floral mutation in Flower #2 and present in all 20 split samples and with the combined data for all 37 de novo mutations of Flower #2 together (R 2 = 79.5, P < 0.0001; Fig 2C ).