Sorbitol promotes the graft healing process in pears.

In heterologous grafts, the sorbitol content exhibited an increasing trend, with significant increases observed particularly in regions R1 and R2.

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KEGG enrichment analysis of the differential metabolites revealed that, on the first day after grafting, in the comparison of the upper scion between self-grafts and heterologous grafts (Y1-S1 vs Z1-S1), most metabolites in amino acid pathways such as ‘Secondary metabolite biosynthesis’, ‘Cysteine and methionine metabolism’, and ‘Valine, leucine, and isoleucine degradation’ showed a decreasing trend, while metabolites in pathways such as ‘Carbon fixation in photosynthetic organisms’ and ‘alpha-Linolenic acid metabolism’ increased.

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