<i>ENO1</i> Regulates Apoptosis Induced by Acute Cold Stress in Bovine Mammary Epithelial Cells.

Establishment of Acute Cold Stress Model and Apoptosis Assessment After acute cold stress treatment, apoptosis rates, cold adaptation responses, and expression levels of apoptosis-related genes in BMECs were analyzed, with the following results: Annexin V-FITC assays revealed a highly significant increase in apoptosis rates at 4, 6, and 8 h post-cold stress compared to controls ( p < 0.01, Figure 1 A,B), with apoptosis severity escalating over time.

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