Genome-wide identiffcation of the WRKY gene family in poplar and the positive role of PsnWRKY95 in response to cadmium stress.

During the Cd treatment, PtWRKY4 , PtWRKY65 , and PtWRKY96 exhibited consistent expression patterns, showing an increasing trend from 0 to 36 hours in roots, while in leaves, their expression continuously increased from 0 to 12 hours, peaking at 12 hours, followed by a decreasing trend from 12 to 24 hours, and then increasing again from 24 to 36 hours.

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Twelve highly significant differential PtWRKYs were selected for RT-qPCR validation of their RNA-Seq results, using Actin as the reference gene ( S1 Table ).

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