Detection of the soil-based fungal DNA by qPCR It was noted that qPCR revealed high sensitivity to detect from 10 3 conidia g-1 soil to 10 6 conidia g −1 soil, besides, the results of qPCR indicated that all primers exhibited an increasing trend in response to the added spore concentrations (Fig. 6 ).
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Genomic analyses of Purpureocillium lilacinum reveal unique DNA regions for developing isolate-specific molecular markers.
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