highly significantP < 0.001
The tow-way ANOVA statistical analysis showed highly significant differences ( P < 0.001) for all studied factors, with an F -value of 6832.140 (Eta squared = 0.997) for the extraction method, 497.409 (Eta squared = 0.986) for the solvent type, and 204.754 (Eta squared = 0.983) for their interaction.