Inhibition of <i>Clostridium perfringens</i> Spore Germination by the Synergistic Effects of the Natural Products Chitosan and Nisin.

However, inhibition was highly significant ( p < 0.01) when a combination of 0.075% nisin and 0.025% chitosan was used ( Figure 3 B), and SM101 spores germinated very poorly upon incubation under this treatment at 37 °C for 60 min, as OD 600 decreased by approximately 10% compared with the initial value, and >95% of SM101 spores remained phase-bright ( Figure 3 B,C).

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