High-throughput proteome integral solubility alteration assay for low cell input using One-Tip.

The one-way ANOVA results confirmed highly significant differences ( p < 0.0001) across the median normalized log2 signal intensities in all five examined subcellular fractions, with the strongest effects observed in the endoplasmic reticulum ( p = 5.6 × 10−14, F = 1134) and mitochondria ( p = 1.8 × 10−12, F = 599), where the addition of DDM substantially increased signal intensity.

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