Gene cloning of S100β and NGF and localization of their expression in the small intestine of broilers of various ages.

In duodenum, the expression level of NGF protein was highly significantly higher at 7 days of age than at 20, 55 and 70 days of age ( P < 0.01), and at 20 days of age than at 55 days of age ( P < 0.001), and at 40 days of age, the expression level of NGF protein was highly significant higher than at 20, 55 and 70 days of age ( P < 0.001), and was significantly higher than at 7 days of age ( P < 0.05) and 70 days of age day-old expression levels were highly significantly higher than 20 and 55 days-old ( P < 0.01).

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The expression level of NGF protein showed an overall trend of increasing and then decreasing, and reached the highest level at 40 days of age.

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