a decreasing trendp < 0.001
No significant decrease but a decreasing trend was observed in 10- and 20-d-old tequila f01792 flies [ Fig. 3 A ; w 1118( ♂ ) (2-d-old) vs tequila f01792 ( ♂ ) t (59) = 4.213 p < 0.001, unpaired t test; w 1118( ♂ ) (30-d-old) vs tequila f01792 ( ♂ ) p = 0.04, Mann–Whitney test].