highly significantP < 0.0001
The correlation analysis demonstrates excellent concordance between RT-qPCR and RNA-seq results, with highly significant Pearson correlation coefficients of R 2 = 0.9216 (P < 0.0001) and R 2 = 0.9323 (P < 0.0001) for the DEGs ( Figures 3C,D ). 3.2 GNA13 is highly expressed in metastasis NSCLC and associated with poor prognosis The pan-cancer analysis was performed to determine whether 17 DEGs were expressed in NSCLC tissues.