Workflow for efficiently isolating microspore cultures of different rice genotypes by optimizing the callus induction medium.

Front Plant Sci · 2025 · PMC12518337 · PMID 41098908

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highly significantP <0.01actually significant

Two-way Analysis of Variance (Two-way ANOVA) showed that there were highly significant differences in callus yield among different CIMs and among different rice genotypes ( P <0.01); in addition, there was also a highly significant interaction between CIMs and rice genotypes on the effect of callus yield ( P <0.01) ( Supplementary Table S1 ).

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