In the spectra of the two-photon excitation action cross sections of these endogenous fluorophores, both FAD and LipDH were observed to have a decreasing trend in their two-photon excitation action cross sections near 1000 nm while the two-photon excitation action cross sections of the others started to decrease at much lower wavelengths [ 24 , 25 ].
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Second harmonic generation of myofibrils exhibits a polarization-resolved "gradient" effect.
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