Whole-genome sequencing bulked segregant analysis uncovered FW7, a Fusarium wilt resistance gene masked by epistasis in octoploid strawberry.

Although the additive effect of FW7 was highly significant in the S 2 family ( p < 0.0001 ), the resistance of individuals homozygous for the favorable FW7 allele and unfavorable FW6 allele ( y ̄ = 1.80 ) was weaker than that observed among individuals homozygous for the favorable FW6 allele ( y ̄ = 1.05 ).

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Although the FW6 × FW7 interaction effect was marginally nonsignificant in the S 1 family analysis (Pr > F = 0.11), the pattern observed among the two‐locus genotypic means substantiated the strength and nature of the epistatic interaction between FW6 and FW7 (Table 2 ; Figure 4 ).

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